This bio-reactor design integrates a hemispherical culture chamber, which maximizes uniform distribution of nutrients and ensures efficient culture growth. The hemispherical shape minimizes dead zones, enhances mixing efficiency, and allows optimal use of space for liquid culture up to 100 mL.

The system is reusable and modular, allowing for easy disassembly, cleaning, and sterilization—critical for long-duration missions. Its gas exchange system, enabled by a PTFE semi-permeable membrane, facilitates oxygen intake and CO₂ release, ensuring a stable aerobic environment while preventing pressure buildup.

The reactor includes dedicated ports (compatible with standard luer lock components) for media intake, sampling, and extraction, making it highly user-friendly. Key components such as the gas exchange membrane cap, culture chamber lid, and PTFE washers ensure a secure and contamination-resistant operation.

Designed with efficiency, reusability, and simplicity in mind, this bio-reactor is optimized for future space missions where reliable, safe, and scalable nutrient production is essential.